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His-tagged NucS was also purified by affinity chromatography and anion exchange purification as described before.
The suspension was centrifuged, and the supernatant was purified by affinity chromatography.
Vn was thereafter purified by affinity chromatography using heparin as matrix.
Here we characterised ScASNase1 using a recombinant enzyme purified by affinity chromatography.
The Tat/AFF/CycT1 complex was purified by affinity chromatography with Ni-NTA resin (Qiagen).
The enzyme substrate complex was purified by affinity chromatography and gel filtration (see Methods).
The biotinylated proteins are captured by affinity and are identified by mass spectrometry.
TIP47 mutants with enhanced or diminished affinities were also selected by affinity chromatography.
Cysteine mutants with altered ligand binding can also be selected simultaneously by affinity chromatography.
Epistemic cultures, shaped by affinity, necessity, and historical coincidence, determine how we know what we know.
The His6-tagged TcGAPDH was purified by affinity chromatography.
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