Furthermore, knockdown of CNK1 impaired the invasion of Matrigel by MDA-MB-231 cells without affecting cell migration, suggesting a defect in proteolytic activity in these cells.
We therefore sought to determine whether inhibition of TRPC6 expression by siRNAs affects cell migration in SCC42B cells.
Further research is needed to reveal the molecular mechanisms by which αB-crystallin affects cell migration.
This may be explained, at least in part, by our observation that SUSD4a affects cell migration, invasion and clonogenic ability.
mTORC2 may affect cell migration by promoting PKCα-dependent phosphorylation of the focal adhesion component paxillin [ 20].
ROCKs can also affect cell migration by limiting the extent of lamellipodial protrusion, as inhibiting ROCK or Rho induces the occurrence of membrane ruffles [ 24].
Together, these pieces of evidence have led to the hypothesis that prenatal organophosphate exposure can affect cell migration by modulating reelin activity, thus potentially contributing to the neurodevelopmental basis of autism (Keller and Persico, 2003).
Migration is a common feature in cancer cells, and therefore we investigated whether or not the presence of P1 affects cell migration by using a flow cytometry-based cell sorting of IGROV1 cells to separate P1-high- from P1-low-expressing subpopulations.
To further investigate the molecular mechanisms by which CYP2E1 gene expression affects cell migration in breast cancer cells, the migration capacity of untreated, ethanol, Bort, APAP [ 40] and the CYP2E1-specific inhibitor CMZ-treated [ 41] MCF7 and MDA-MB-231 cells was monitored.
To explore the mechanism by which miR-215 negatively affects cell migration and invasion, we examined the effect of miR-215 transfection on SIP1/ZEB2 (smad-interacting protein 1/zinc-finger E-box-binding homeobox 2) protein expression, which is a direct predicted target of miR-215 (Supplementary Table 1).
