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Á dose-response curve of inhaled APC was prepared by administering increasing concentrations of the drug to three mice and measuring lung elastance H and airway resistance RN every 10 seconds for 30 minutes.
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The concentration of IGF-1 was constant (200 ng/mL) whereas HGF was administered at increasing concentrations from the atrium to the border zone (50, 100, 200 ng/mL).
For the determination of the IC50-value inhibitors were administered with increasing concentrations to the enzyme and its substrate.
Serotonin was administered in stepwise increasing concentrations from 10−10 to 10−5 M. In preparations from normal pregnancies, serotonin 10−7 M, was administered before and 30 min after the start of a continuous CsA infusion (1.0 mg/l).
To assess airway hyperreactivity, the mice were challenged with metacholine in physiological saline generated with an in-line nebulizer and administered directly with increasing concentrations (0, 6.25, 12.5 mg/mL) for 20 seconds.
This binding was inhibited by increasing concentrations of unlabeled hPL (half-maximal concentrations, 2.2 and 3.4 nmol/L, respectively).
Increasing concentrations of 4,4′-DDE administered to the culture wells produced an increasing trend in PHA-induced proliferation.
Increasing concentrations of androgen were cumulatively administered directly into the bath for 5 minute intervals.
Using increasing concentrations of ethanol followed by propylene oxide, the cells were embedded in epon resin.
(c) Affinity measurements by microscale thermophoresis of labeled GST-Pmp23 binding to increasing concentrations of PBP2x.
Here, increasing concentrations of PEA are presented.
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