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Our results support the modification of the 6-d synchronization protocol by administering a single high dose of PGF 6 d after GnRH (with the subsequent reduction in labor resulting from reduced handling of animals) without detrimental effects on the luteolytic response of dairy cows and preovulatory diameter of the dominant follicle compared with the original protocol.
After the third afternoon sample, we conducted a hypothalamic response test by administering a single intravenous injection of NMA (Sigma) at a dose of 40 mg/kg (Gore et al. 1996; Nyberg et al. 1993).
Type 1 DM was induced in male Sprague Dawley rats (200 224 g body weight) by administering a single intraperitoneal injection of STZ (65 mg/kg body wt) prepared daily in citrate buffer pH 4.5 for maximal stability.
LPS injury was performed as a two-hit model by administering a single bolus of 1 mg/kg body weight intraperitoneally 24 hours prior to the experiment, followed by a constant intravenous infusion of LPS (1 mg/kg/hr) during the 6-hour experimental period.
In the pregnant rabbit, the study was performed on day 28 of gestation by administering a single dose of ZNM orally (by gavage) at a dose of 10 mg/kg in a freshly prepared suspension (20 mg/mL ZNM in 0.5% CMC).
Since DNA analogues are commonly used to show actively dividing stem cells and their lineages (Eisenhoffer et al., 2008; Snippert and Clevers, 2011), we labeled the proliferating cells by administering a single pulse of EdU, and monitored EdU+ cells in RFP+ cells.
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T1DM was induced in the experimental rats 11 by administering a single-dose intraperitoneal (IP) injection of STZ (60 mg/kg) (Sigma-Aldrich Co, St Louis, MO), dissolved in distilled water.
To evaluate its effect in myocardial repair, we induced a myocardial infarction (MI) by coronary artery ligation and administered a single intramyocardial dose of pyrvinium.
As dysferlin absence causes a defect in membrane repair, we tested the ability of single fibers from transplanted mice to reseal the membrane after a focal damage produced by a laser, as previously described by Bansal et al. We administered a single injection of 5 × 10 GFP-labeled, C57-J1 cells into tibialis anterior, extensor digitorum longus and gastrocnemius of 5-month-old SCID/BlAJ mice.
Optimization was carried out using acslXtreme parameter estimation tool kit, using ClO4− kinetic data reported by Yu et al. (2002), who administered a single intravenous bolus dose of 3.3 mg/kg ClO4−.
NR-INF-02 was evaluated for its acute oral toxicity by administering as a single oral dose to albino Wistar rats.
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