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Some of this variability could be explained by adherence with the intervention, with larger effects in studies with higher adherence, but some variability remained which we were unable to explain despite multiple subgroup analyses.
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However, we also explored the effectiveness by adherence subgroups, with high adherence defined as ≥80%%.
Cells expressing the immature cell surface marker, CD34 (My10), were isolated by immune adherence with the "panning" technique or immunomagnetic microspheres and were shown to be strongly positive for c-myb protein expression in an immunoperoxidase assay.
Process evaluation will be assessed by calculating adherence with intervention (mean number of sessions completed, average time of intervention delivery for face-to-face and telephone counseling sessions).
Monocytes were enriched by adherence and were cultured with granulocyte monocyte colony-stimulating factor and IL-4 (>90% CD1a+ DCs at day 5).
However, by mid-2008, high adherence with diagnostic results was achieved and continues to be sustained on a national scale.
Single-cell splenocytes suspensions were twice depleted of Ig+ B cells by adherence to culture plates coated with anti-Igμ mAb and anti-Igκ mAb for 60 min at room temperature.
Single-cell splenocytes suspensions were twice depleted of CD8+ T cells by adherence to culture plates collated with anti-CD8 mAb (clone 3.155) for 60 min at room temperature.
Blood samples were collected from healthy donors and monocytes were isolated by adherence and further incubated together with live planktonic or biofilm forming strains of P. aeruginosa or S. aureus for 5 hours; the cytokine content was measured by ELISA.
On-the-job preparation entails participation in work activities at a firm, which includes a systematic regimen characterized by adherence to training regulations and interactions with trainers or masters who guide apprentices through the realistic components of the apprenticeship (Ashton and Green [1996]; Kutscha [2000]).
The anonymised EHR data are available for secondary research via CRIS35 subject to approval by the CRIS Oversight Committee in adherence with strict patient-led governance35.
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