Sentence examples for by additional two from inspiring English sources

After 45 min of incubation at RT cells were again washed three times with PBS/gelatine followed by additional two wash steps in PBS.

The full abstracts of potentially eligible studies were then scanned by additional two reviewers (PC, JT) who worked independently to select potentially relevant full-text articles.

In contrast, in fluorescent pseudomonads REPs, palindromes are flanked by additional two or three nucleotides on both sides and the length of the palindromes is significantly shorter (Table  1, Table  2).

DS was imposed by withholding water and allowing the pots to reach a SWC equal to 12.5%, while HS was imposed progressively exposing the plants for two days at 30°C followed by additional two days at 34°C, and subsequently to 40°C.

The accuracy of the proposed equation was validated by additional five groups of BFRP tubes with different slenderness ratios.

Compounds 3 and 4 are built from the disupporting sandwich-type polyoxoanions 1 and 2, linked by additional four Mn2+ ions to construct a 1D ladder-like chain-like structure, which is rarely observed in the POM chemistry.

Cells were then cultured for another 24 hours and 20 µL MTT (5 mg/mL) was added in each well, followed by additional four hour incubation.

Incubation of the secondary antibody was processed at 37°C for 45 min followed by additional three washes with HBSS supplemented with 2% FCS.

Next, brains were post-fixed in 4% PFA in PBT for 30 min. After fixation brains were washed two times 5 min each in PBT containing 0.1% DEPC followed by additional three washes in PBT for 5 min each.

This was followed by additional eight cycles with 53 °C annealing temperature to facilitate the annealing of the fluorescent dye-labelled M13 primer, and a final extension step of 10 min at 72 °C.

Our intervention will increase PA by an additional two hours per week by introducing brisk walking into curriculum subjects.