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These results point to the possibility to facilitate the start-up under high CH3COO− concentrations during anaerobic digestion by addition of specific adapted inocula.
Salting in, however, requires two-state water to explain how the solubility of a protein can be enhanced by addition of specific other small solutes Surfaces, also, induce osmotic pressure gradients in two-state water, since chaotropic moieties on the surface tend to induce HDW and kosmotropic moieties tend to induce LDW.
NF-κB (p65) is detected by addition of specific primary antibody directed against NF-κB (p65).
Cells were infected by addition of specific numbers of sporozoites (typically 30 000), followed by centrifugation at 1700 g for 7 min at 37°C.
Furthermore, the enzyme activity can be further improved by increasing the thermostability by addition of specific ions in the growth media [ 31].
A study in isolated rat cardiac myocytes demonstrated that gap junctional intracellular communication (GJIC) disturbance, by either silencing the gene for Con43 or by addition of specific drugs, such as heptanol, induced a significant reduction in Ca2+ transients, suggesting that Con43 may be involved in the regulation of basal Ca2+ signalling in these cells (Li et al., 2012).
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CTL effector (E) cells were stimulated by addition of the specific peptide, target (T) cells pulsed with the specific peptide (E∶T 10∶1), or by PMA (10 ngm/ml) ionomycin (1 µg/ml) (Sigma).
Similarly, inactivation of cGKII by either introducing a point mutation in the catalytic domain (cGKII-D576A [21]) or by addition of a specific pharmacological blocker KT5823 abolished the inhibitory cGKII effect on WT HCN2 to a similar extent as the S641A mutation (Fig. 4H, sixth and seventh column).
This effect was reverted by addition of a specific inhibitor of TGFβ I receptor thus demonstrating a TGFβ dependent effect of core on EMT development.
In this study, we showed that Gal-1 induces apoptosis both in vitro and in vivo, a process that was prevented by addition of the specific disaccharide lactose.
Furthermore, in the present study, effect of SAF on different sperm parameters, namely, Mg2+ dependent ATPase and apoptosis, was studied and receptor-dependent interactions can be confirmed if they are competitively inhibited by addition of the specific receptor component.
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by administration of specific
by apoptosis of specific
by secretion of specific
by addition of catalytic
by light of specific
by addition of phosphoric
by implementation of specific
by recruitment of specific
by comparison of specific
by isolation of specific
by acquisition of specific
by deamination of specific
by association of specific
by introduction of specific
by identification of specific
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