Sentence examples for by addition of sample from inspiring English sources

Bound proteins were then eluted by addition of sample buffer and assessed by immunoblot analysis.

Following 2 h further incubation at room temperature samples were prepared for SDS-PAGE by addition of sample buffer containing 5% beta-mecaptoethanol and immunoblotted as described above.

The reactions were stopped by addition of sample buffer and 20 100% of the reactions were analyzed by SDS-PAGE.

Reaction was quenched by addition of sample buffer to 1X and boiling at 95° for 5 minutes.

Following incubation with the splicing mix; bound proteins were eluted by addition of sample buffer, heated for 5 min at 90°C, and separated on 12% SDS-PAGE.

The reaction was stopped by addition of sample buffer (10% glycerol, 50 mM Tris HCl, pH 6.8, 3% SDS and 5% b-mercaptoethanol), boiled, and separated on a 12.5% SDS-PAGE gel.

The reaction was stopped by addition of LDS sample buffer with sample reducing agent (Invitrogen).

Samples were denatured by addition of SDS sample buffer, boiled briefly, and run on a 12% SDS PAGE.

Samples were prepared for electrophoresis by addition of LDS sample buffer and reducing agent (Invitrogen), followed by heating (65°C, 5 min).

Samples were incubated for 20 min at 37°C and reactions were stopped by addition of SDS sample buffer.

Human brain samples were prepared as 20% homogenates and equal protein concentrations then prepared for SDS PAGE by addition of SDS PAGE sample buffer and heating to 100°C.