Sentence examples for by addition of extracellular from inspiring English sources
As the incidence of ductal cells (based on immunostaining for CK7) is uncommon in Dex-treated cultures, we tried to enhance the number of ductal cells by addition of extracellular factors.
Furthermore, cells from S100A9 deficient mice display reduced TNFα production when stimulated with LPS, a deficiency that could be restored by addition of extracellular S100A8/A9 [ 29].
Indeed, the Asp447Glu mutation leads to a rapid and complete decrease in ionic current (Molina et al., 1997, 1998), and this loss in conductance can be drastically slowed by addition of extracellular tetraethyl ammonium (TEA).
In whole-cell patch-clamp recordings from DT40 cells expressing these IP3R, intracellular IP3 again activated cation channels in the PM, but their γ and open probability (Po) were both increased by addition of extracellular, but not intracellular, αBgtx (65).
To rapidly reduce the C-XIAP-C concentration, we selectively permeabilized the plasma membrane of MCF-7 cells on stage of the microscope by addition of mild extracellular concentrations of digitonin (see Materials and Methods).
A high concentration of BDE-47 (20 μM) enhanced the Ca2+ transient amplitude triggered by bolus addition of extracellular K+, whereas other PBDE congeners lacked significant influence (Dingemans et al. 2010).
By contrast, addition of extracellular insulin (20 nmol/l) led to inhibition of glucagon secretion in control and Pask-silenced alpha-TC1-9 cells, indicating (1) the presence of distinct glucose and insulin signalling pathways in the alpha cell and (2) that the insulin signalling pathway in Pask-silenced alpha-TC1-9 cells was intact (Fig. 4b).
In these cells the loss of tagged cyclin C protein and mRNA can be induced by addition of high concentrations of extracellular cAMP similar to those experienced in the mound (Figure 2C).
Our results suggest that efficient ethanol production by SSF could be achieved by engineered yeast capable of fermenting cellobiose without addition of extracellular β-glucosidase, leading to economic production of cellulosic ethanol.
This fluorescence can be attenuated by addition of exogenous catalase to the extracellular media, indicating that H2O2 is one of the formed oxidants in this system.
Addition of extracellular glutamate depletes the cells of glutathione (GSH) by blocking the glutamate−cystine antiporter system xc−.
