Sentence examples for by addition of buffer from inspiring English sources

Protein concentrations were measured using the Biorad DC protein assay and were equalized by addition of buffer.

Red blood cells were lysed by addition of buffer (pH 7.2) containing 150 mM NH4Cl, 1 mM KHCO3, and 0.1 mM EDTA; debris were removed by straining.

For controls, each sample was measured individually at these same concentrations, and brought to the same final volume by addition of buffer.

DNA synthesis was initiated by addition of buffer O containing 75 μM dTTP and 0375 μCi [α-P] dATP or 75 μM.

Immobilized GST-Rio2 was incubated with buffer alone or 0.5 µM Slx9, followed by addition of buffer, 50 nM Crm1-His6, or the stepwise addition of 0.2 µM His6-RanQLGTP and 50 nM Crm1-His6 as depicted in (A ).

Hypotonic swelling was stopped by addition of buffer (525 mM mannitol, 175 mM sucrose, 12.5 mM Tris-HCl pH 7.5, 2.5 mM EDTA) and cells ruptured by Dounce homogenization.

Then buffer was removed and oocytes were fixed by addition of fixation buffer as described above.

Bound proteins were eluted by addition of extract buffer supplemented with 1 mM vanadate followed by extract buffer supplemented with 10 mM reduced glutathione.

Bound proteins were eluted by addition of extract buffer supplemented with 1 M NaCl followed by extract buffer supplemented with 10 mM reduced glutathione.

Bound proteins were then eluted by addition of sample buffer and assessed by immunoblot analysis.

Following 2 h further incubation at room temperature samples were prepared for SDS-PAGE by addition of sample buffer containing 5% beta-mecaptoethanol and immunoblotted as described above.