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The optimum reagent concentration was determined by adding various volumes of 4 × 10-3 M eosin Y solution.
For each acid digestion analysis an acid washed 100 ml tall form quartz digestion beaker covered with watch glass was used and by adding various volumes (25 30 ml) of HNO3 and 3 5 ml of H2O2 (added in five steps).
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Figure 6 The fluorescence intensity change of different QDs when added various volumes of 0.03% H2O2 solution.
They were initiated by the addition of enzyme (typically 20 nM) and quenched at various times by adding 5 volumes of 90% formamide, 10 mM EDTA, 1× Tris Borate EDTA buffer, and 0.1% bromophenol blue.
Intracranial volume was calculated by adding gray matter volume, white matter volume, and CSF space volume.
Precipitation was initiated by adding small volumes of ethanol.
Then, mixed solutions were adjusted to a constant final volume (5 mL) by adding additional volumes of deionized water.
After that, DNA was precipitated from supernatant by adding 2.5 volumes 100% ice-cold ethanol.
The latter was done by adding 6 volumes of ethylacetate.
The 2,2,2- trifluoroethanol (TFE) experiments were performed by adding increasing volumes of TFE (JT Baker) added to the samples.
After the compounds were completely dissolved, two volumes of Cremophor EL were added and final dosing solution was prepared by adding six volumes of 20% hydroxypropyl β-cyclodextrin.
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