Sentence examples for by adding two volumes from inspiring English sources
DNA was precipitated by adding two volumes of isopropanol followed by suspension in TE (10 mM Tris, 1 mM EDTA, pH 8).
In brief, proteins were denatured by adding two volumes of 8 M urea (Sigma-Aldrich).
The reaction was terminated by adding two volumes of 0.5 N NaOH.
The RNA was precipitated by adding two volumes of isopropanol and incubating at room temperature for 10 min.
DNA was precipitated by adding two volumes ethanol absolute and pelleted by centrifugation (3,000 rpm for 10 min).
After centrifugation crude extracellular glycoproteins were isolated by precipitation from the supernatant by adding two volumes of 96% (v/v) ethanol and subsequent centrifugation.
Total carbohydrates were recovered from the flow-through by adding five volumes of ethanol, followed by overnight incubation on ice and centrifugation at 18,000 g for 30 min.
Dissolved hemicellulose fraction was removed through precipitation by reducing the pH of filtrate up to 5.5 with 5 N HCl followed by adding three volumes of 95%% ethanol.
In order to purify the DNA from the gel, the MinElute Gel Extraction Kit (Qiagen, Valencia, CA, USA) was used by adding six volumes of buffer QG to the gel pieces and vortexing the mixture until the gel was dissolved (about 5 min).
