Sentence examples for by adding three volumes from inspiring English sources

Dissolved hemicellulose fraction was removed through precipitation by reducing the pH of filtrate up to 5.5 with 5 N HCl followed by adding three volumes of 95%% ethanol.

The solution was precipitated for 6 h by adding three volumes of distilled water (v/v).

The eluted protein was precipitated by adding three volumes of ice cold acetone and incubating overnight at -20°C.

RNA was isolated from conditioned medium by adding three volumes of Trizol LS (Invitrogen) to one volume of conditioned medium and completing the extraction according to the manufacturer's protocol.

The reaction was stopped by adding three volumes of cold ethanol followed by incubation at 4°C for 15 min, and the protein material was then centrifuged at 10,000 g for 10 min at 4°C.

GAGs were precipitated by adding three volumes of 96° ethanol and 4% potassium acetate; the precipitate was dissolved in 0.4 M guanidine chloride with 50 mM sodium acetate at pH 5.8.

DNA was precipitated by adding two volumes of isopropanol followed by suspension in TE (10 mM Tris, 1 mM EDTA, pH 8).

In order to purify the DNA from the gel, the MinElute Gel Extraction Kit (Qiagen, Valencia, CA, USA) was used by adding six volumes of buffer QG to the gel pieces and vortexing the mixture until the gel was dissolved (about 5 min).

In brief, proteins were denatured by adding two volumes of 8 M urea (Sigma-Aldrich).

The reaction was terminated by adding two volumes of 0.5 N NaOH.

DNA was precipitated by adding two volumes ethanol absolute and pelleted by centrifugation (3,000 rpm for 10 min).