Enzyme inactivation is done by adding the Neurobasal medium supplemented with 10% heat inactivated fetal bovine serum, GlutaMAX 1× and 1% Pen-Strep (dissociation medium).
Transfection was stopped after 5 h by adding the same volume DMEM/F12 supplemented with 20 % FBS.
The search is supplemented by adding the reference lists of recent reviews in simulation-based medical education and with authors' records of studies published in the period of interest.
The questions on trust and reciprocity will be supplemented by adding the phrase "in this residential home" to situate the measures of trust and reciprocity in that particular residential environment.
Trypsin solution was then inactivated by adding the same volume of BM supplemented with human serum to flasks, and the resulting cell suspension was replated in new cell culture flasks.
The Fol protein database used for the analysis was obtained from Fusarium Comparative Genome website (http://www.broadinstitute.org/annotation/genome/fusarium_group/MultiHome.html) and supplemented by adding the sequences of known Six proteins that are not annotated in the public database.
Total daily nutrient intakes were calculated by adding the amounts from multivitamins and specific supplements to the intakes from food.
Total intake was calculated by adding the nutritional content of the oral supplement.
They supplemented the investigation by adding the sensation of pressure and two-point discrimination tests.
Add the "supplement slurry" to that and mix again.
