Sentence examples for by adding the substrate from inspiring English sources

Initiate the reaction by adding the substrate stock solution (5 μl) and seal the HPLC vials.

At the time of activation, the pro-TPPI was incubated with 25 µM NBS, 10 mM CaCl2 or 10 mM EGTA for 15 min. Following this preincubation, the pro-TPPI was activated by autocatalysis in 50 mM ammonium formate buffer, pH 3.5 for 5 min at 37°C, and finally TPPI activity was measured by adding the substrate GFFL-AFC.

After further washes and 45 min incubation with ExtrAvidin-alkaline-phosphatase at 1 µg/mL (Sigma), the IFN-γ producing cells were visualized by adding the substrate BCIP-NBT (Five-bromo-4-chloro-3-indolylphosphate/nitroblue tetrazolium; Sigma) in 100 mM Tris, 100 mM NaCl, 5 mM MgCl2, pH = 9.5 for 30 min. Results were expressed as the number of spot-forming cells (SFC) per 106 input cells.

Bound antibody was revealed by adding the substrate 3,3-diamino-benzidine.

The bound antibody was revealed by adding the substrate: 3,3'-diamino benzidine.

The transfected leaves were assayed 2 days after agroinfiltration by adding the substrate (10 mM luciferin).

Ten microliters of the supernatant was diluted with 170 µL activity assay buffer (100 mM phosphate buffer, pH 6, containing 1 mM EDTA, 1 mM DTT, and 1 mM β-mercaptoethanol), and the activity assay was initiated by adding the substrates, 3-HPA and NAD+.

Dilutions of the insoluble Zera-Xyl suspension were submitted to a xylanase activity assay by adding the synthetic substrate and measuring the fluorescent product.

The assay was developed by adding the enzyme substrate (O-phenylenediamine diluted to 0.3 mg/ml in Phosphate-citrate buffer in the presence of hydrogen peroxide).

After washing away unbound ACE, plate-bound ACE activity was measured by adding the ACE substrate (Hip-His-Leu or Z-Phe-His-Leu) directly into wells [30].

Color reaction was developed by adding the phosphatase substrate until dark bands, appeared in comparison to the positive control.