Sentence examples for by adding the stop from inspiring English sources

After adding the assay buffer (PNP-α-Gal + CH3COONa) to the supernatants and incubating at 30 °C for 60 min, the reaction was terminated by adding the stop solution (Na2CO3).

The reaction was terminated after 20 min by adding the stop solution.

After 1 h, the reaction was quenched by adding the stop buffer.

The reaction was stopped by adding the stop solution, and after adding the complete developing solution the mixture was incubated for another 15 minutes at room temperature.

After further washing, a substrate solution was added to the plates to initiate a reaction, which was stopped after 15 min by adding the stop solution (HCl).

The reaction was halted by adding the stop solution in the plate reader at 450 nm and analyzed using a Biotech EL808 ELISA Reader (BioTek, Inc., Winooski, VT).

The enzymatic reaction was terminated by adding the stopping solution and the optical density was read at 450 nm using a Tecan Spectra plate reader.

The enzymatic reaction was terminated by adding the stopping solution and the optical density was read at 450 nm using a Tecan Spectra (Austria) plate reader.

The reaction was stopped by adding the Dicer stop solution and 22 bp products were detected using 3% agarose gel electrophoresis [ 37].

The digestion was stopped by adding the 10× exonuclease stop buffer having 0.5 mg/ml proteinase K, 200 mM Tris-HCl pH8, 50 mM EDTA and 5%SDS.

The reactions were started by adding the enzyme and stopped by boiling.