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More generally, this shared genotype is the one genotype obtained from a pair of adjacent genotypes G1 and G2 in V(n) by adding the reaction to G1 that it does not share with G2, or vice versa.
Liver microsomes (100 μg protein) were treated with 0.25% Triton-X at 37°C for 5 min, followed by adding the reaction mixture [40 mM Tris-HCl buffer (pH 7.4), 0.8 mM MgCl2, and 0.2 mM 1-naphthol].
The activity was measured by adding the reaction mixture in a 1 to 10 ratio to Ultima gold scintillation liquid (Perkin-Elmer Life Sciences, Boston, MA) and scintillation counting in a model 1600 TR Tri-Carb liquid scintillation counter (Packard Instrument Company, Meriden, CT).
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We have updated our biochemical thermodynamic database by adding the reactions of the pentose phosphate pathway.
Here an updated database is developed by adding the reactions of the pentose phosphate pathway into the original database.
A database of thermodynamic properties is developed, which extends a previous database of glycolysis and tricarboxylic acid cycle by adding the reactions of the pentose phosphate pathway.
Labeling of proteins with two fluorophores was completed in a single reaction step, by adding the reactive dye molecules together into the reaction mixture.
This was done by setting to zero the flux from crotonyl-CoA to propionyl-CoA and by adding the ICL reaction.
Of the 11 category I solutions, five fixed false negatives by adding the deleted model reaction back in from the universal reaction list.
Fast-binding inhibition was determined by adding the inhibitor into the reaction system before FAS initiated the reaction.
Another solution suggested that false negatives for Δ aspC strains could be corrected by adding the existing model reaction ASP1DC (aspartate 1-decarboxylase) in reverse.
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