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Monsanto engineered resistance by adding the gene for a similar microbial enzyme that isn't affected.
This vector was constructed by adding the gene cre amplified from pNZ7125 [17] into plasmid pCU1 and replacing the BglII restriction site with a SmaI site upstream from cre.
As many epilepsy genes involve ion channels she could further reduce the number of genes by adding the Gene Ontology ID for ion transport (GO 0006811) into the highlight these GO IDs fields and restrict the search to those carrying this GO ID or a subclass.
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What's more, by adding the genes for all the other viral structural proteins, the team could boost virus production more than tenfold.
To search for the genes related to the Tn 1549-like conjugatransposonsosons (CTns), we also constructed a modified Ref-DB by adding the genes on Tn 1549 from Enterococcus faecalis strain BM4382 (AAF72340-68) and on CTn2, CTn4, and CTn5 of Clostridium difficile strain 630.
The final responsive gene modules were constructed by adding the intermediate genes to the DE gene groups found in the clustering step.
Here we presented a new formulation of this task: a group of closely-connected and co-expressed DE genes in the gene network are regarded as the signatures of the underlying responsive gene modules; the modules can be identified by finding the signatures and then recovering the "missing parts" by adding the intermediate genes that connect the DE genes in the gene network.
Results for the first phase, the replication set and for the joint analysis are shown in table 2. We calculated Meff values for each candidate gene separately and for the whole study (by adding the individual gene Meff values; details are shown in supplementary table S3).
Several teams have been trying to develop a one-time injection of a virus that defends brain cells by adding the GDNF gene, with some initial success.
It was also reported in 2001 that both Sf9 and High Five cells were engineered to produce sialylated proteins by adding the ST6GAL1 gene (Hollister and Jarvis 2001; Breitbach and Jarvis 2001).
A second pIRES vector was constructed by adding the coluxD gene to MCS A via the unique NheI and EcoRI restriction sites and the addition of cofrp to MCS B using the unique SalI and NotI restriction sites.
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