Exact(1)
The total amount of DNA was adjusted to 2 µg by adding the empty vector.
Similar(59)
Total DNA concentration in each experiment was maintained by adding the appropriate empty vector to the DNA mixture.
The amounts of DNA in each of these experimental conditions were equalised by adding the corresponding empty vector.
The total plasmid DNA per dish was kept constant by adding the appropriate amount of empty expression vector.
Total amounts of transfected DNA per cell dish varied depending on the experimental protocol, but the total DNA content per dish within the experiment was kept constant by adding the appropriate amount of empty expression vector.
In similar reporter assays, HEK-TLR3 cells were transfected with reporter plasmids for IFN-β, NFκB, CCL5, and the internal control β-gal, and expression plasmids pcDNA3-I329L or pcDNA3 (negative control), keeping the total amounts of DNA and equimolar ratios constant in all assays by adding the appropriate amount of empty vector.
For co-transfection, we equalized the total DNA amounts by adding the appropriate amount of pCDNA3.1 empty circular vector (Invitrogen).
The total DNA used in each transfection was adjusted by adding the appropriate amount of pcDNA3 empty vector.
The amount of nucleic acid was held constant across conditions in every experiment by adding the respective negative controls (negative control siRNAs/mimics or empty vector).
We found that by adding the spermine before the IO-MHPC, we were able to surface load IO-MHPC in the presence of high concentrations of empty micelles.
Total DNA for each plate was adjusted to the same amount by adding relevant empty vector.
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