Sentence examples for by adding the buffer from inspiring English sources

The enzyme reactions were stopped by adding the buffer with higher pH values for maintaining p-NP released in a phenolate form as described above.

Hydration process of the dry lipid film was accomplished by adding the phosphate buffer solution (PBS) of pH 7.4, which resulted in liposome suspension.

Formed blue formazan crystals were dissolved by adding the solubilization buffer (100 µL/well) and placing the plate on an orbital shaker overnight.

The dry lipid film was hydrated by adding the PBS buffer and carrying out at least 5 freeze-thaw cycles between liquid nitrogen and room temperature and then homogenizing several times during the thawing steps by vigorous vortexing.

The dry lipid-peptide film was hydrated by adding the PBS buffer and carrying out at least 5 freeze-thaw cycles between liquid nitrogen and room temperature and then homogenizing several times during the thawing steps by vigorous vortexing.

After 1 h, the reaction was quenched by adding the stop buffer.

Baseline staining was obtained by adding the appropriate buffer to the cells instead of primary antibody.

The mRNA was interrupted into short fragments by adding the fragmentation buffer provided by the manufacturer (Illumina RNA-seq kit, part no. 1004898).

Baseline staining was obtained by adding the appropriate buffer to the cells instead of primary antibody, and by staining with non-relevant isotype-treated antibodies.

The reaction was stopped by adding the termination buffer, which contained a protease that removes amino acids specifically from the non-phosphorylated substrate and results in the production of highly fluorescent rhodamine 110.

Experiments were initiated by adding the assay buffer mix [20 mM Hepes (pH 7.4), 3 mM MgCl2, 100 mM NaCl, 10 μM GDP and 0.2 mM ascorbic acid] containing 50 nCi of [S]GTP[S] in the presence or absence of ligands to defined amounts of membranes.