Sentence examples for by adding the alkaline from inspiring English sources

Bound probes were later visualized by adding the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) then nitroblue tetrazolium (NBT).

Following another PBST wash, plates were developed by adding the alkaline phosphatase yellow (pNPP) liquid substrate for ELISA (Sigma).

Plates were then washed with PBST, and the signal was developed by adding the Alkaline Phosphatase Yellow (pNpp) Liquid Substrate System for ELISA (Sigma).

Both ELISAs were developed by adding the alkaline phosphatase substrate p-nitrophenyl phosphate (Sigma), and the OD was monitored at 405 nm using a MRX Revelation ELISA Reader DYNEX Technologiess, Chantilly, VA).

After five washings, color was developed by adding the alkaline phosphatase substrate (p-nitrophenyl phosphate) at 100 μL/well and incubating the plates for 60 minutes at room temperature in the dark.

We have stopped the enzyme reaction by adding cold alkaline buffer containing EDTA (pH 8.0) in order to quench the released p-NP into para-nitrophenylate form allowing the colorimetric measurement of the latter at a standard wavelength of 410 nm.

In order to improve the thermal stability and carbon-deposition resistance of the Ni/γ-Al2O3 during the reforming of methane with carbon dioxide, the catalyst was modified by adding an alkaline earth (CaO).

TB assay was developed by adding an alkaline phosphatase-conjugated detection antibody and substrate.

Following a wash with PBST, the signal was developed by adding Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System For ELISA (Sigma).

It has to find a way to dispose of it or neutralize it by adding alkaline materials, without creating more environmental problems or raising costs.

Initial formaldehyde/phenol mixtures were fixed to pH=8.0 by adding triethylamine as alkaline catalyst.