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FICindex was obtained by adding the FICs of P60 and caffeine.
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Then, the synergistic effect was evaluated by calculating the FICindex (FICI) for each combination, by adding the individual FIC values.
The FIC index was calculated by adding both FICs and this was interpreted in the following manner: a synergistic effect when its value is ≤0.5; no interaction when the value falls within the >0.5 to 4.0 range; and antagonistic effect when it is >4.0.
The FIC index (FICI, the sum of the FICs) was calculated as described by Meletiadis et al. 2005 [ 9].
FIC is the MIC in combination divided by the MIC of the individual drug and FICI is the sum of the FICs of individual drugs.
FICI is calculated as the sum of the FICs of each agent.
The cumulative fractional inhibitory index (∑FIC) was calculated as the sum of the FICs of drug A and drug B to evaluate interaction profiles.
The AvrB structure is also the top hit using doc as a query, followed by the fic domains.
The inhibitory glutamate in aforementioned helix obstructs the ATP binding site and hence inhibits AMPylation by the Fic domain.
The response parameters were determined, and the IC50 of the combined compounds were expressed as sums of the FICs (ΣFICs) of each added compound.
The presence of pNH4 and the identification of B. henselae were verified from the blood agar colonies by quantitative PCR targeting the Fic gene (specific to pNH4 only) and the pap31 gene (specific to B. henselae only) [21].
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