The luciferase protein is destabilized using both a PEST protein degradation sequence as well as a CL1 sequence, and mRNA is destabilized by adding five tandem AUUUA repeats into the 3′UTR.
The GB male and female tandem pairings of Neil Fachie and Craig Maclean and Sophie Thornhill and Rachel James added tandem sprint golds to the kilos titles both pairs won on Friday.
Vertical scaling was achieved by adding more copies of the shRNA in tandem onto the 3′ UTR of the reporter gene; up to four copies were added, separated by spacer sequences.
After centrifugation (3,600 g, 15 min), the upper layer was isolated by decanting it into a PP centrifuge tube and acidified by adding 20 μL of FA prior to the tandem solid-phase extraction (SPE) procedure (Fig. 2a).
If you wish to use an aspirator set it up such that you have 2-containers in tandem and protect the house vacuum by adding liquid filters to the tubing.
Simulated data are obtained by adding mutations under a stepwise mutation model [ 56] for short tandem repeats (STRs) and an infinite sites model [ 57] for sequence data.
In the present study, a tandem demetalization desilication strategy was developed to enhance porosity of ATP by adding NH4F followed the HCl treatment, for the first time.
Alternatively, bursts may be smoothed by adding independent or anticorrelated transgene copies [33], [38], [91] in place of fully correlated copies that arise from tandem integration and coamplification.
By adding Cromartie, a Pro Bowl player in 2007 who is in the last year of his contract, the Jets have, on paper, one of the best cornerback tandems in the N.F.L.
