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This is done by adding sulphate to them.
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George is convinced that by adding iron sulphate to the oceans, he can stimulate plankton blooms and so suck enough carbon dioxide out of the atmosphere to offset human emissions from burning coal and oil.
Proteins were precipitated from the supernatant by adding solid ammonium sulphate to 70% saturation and then pelleted by centrifugation.
Synthetic textile effluent was prepared by adding calcium sulphate (130 mg/L), calcium chloride (312 mg/L), sodium bicarbonate (520 mg/L), magnesium sulphate (316 mg/L), crystal violet solution (5 mL) and Rhodamine dye (1 mg/L) (Ranganathan et al. 2006; Ramesh Babu et al. 2007).
De novo sulphated glycosaminoglycan and collagen synthesis were measured by adding [S]-sulphate and [H]-proline to the media, respectively.
Briefly, plasma was diluted 1∶4 with PBS and VLDL and LDL precipitated by adding Dextran Sulphate Na-salt (1 mg/ml) and MgCl2 (0.09 M), for 1 hour at +4°C.
Supernatant of culture was also collected and precipitated by adding ammonium sulphate at a final concentration of 75% (w/w).
The protein present in cell free supernatant was precipitated by adding ammonium sulphate at a final concentration of 75%.
When the OD600 reaches ~1.7, the cells were harvested and the protein in culture supernatant was concentrated by adding 75% ammonium sulphate.
In vitro tissue lipid peroxidation was induced by adding H2O2 and ferrous sulphate (FeSO4.7H2O) at a final concentration of 1 mM and 0.5 mM, respectively, in both test and control reaction mixtures.
A different solution was prepared by adding 0.5 g of cadmium to 2 mL of an aqueous 5% cupric sulphate (CuSO4) solution and mixed for 10 min.
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