Sentence examples for by adding subsequent from inspiring English sources

Each sample was read four times by adding subsequent 5-µl aliquots of a formaldehyde-fixed cell suspension to the same well.

The reaction is practically stopped by adding a subsequent volume of de-ionized water.

The sample was either quenched instantly by adding −20°C acetonitrile, subsequent to substrate (C16:0-CoA) addition (Fig. 1, A and B), or was incubated for 5 minutes at 37°C (Fig. 1, C and D).

PMN were pre-treated with dapsone in different concentrations (1.6 100 μmol) subsequently by adding Ca ionophore A 23187 and subsequent incubation.

Non-linearity was tested by adding quadratic terms of subsequent conditional BMI SDS scores to the models (at a type 1 error rate of 0.05).

Subsequent oxidization by adding Ce SO4 2 will regenerate vesicles.

Protein expression was induced by adding 0.4 mM IPTG and subsequent culturing at 25°C overnight with shaking.

Static P and MAS H NMR spectroscopy investigations were carried out on MLV preparations made by co-dissolving lipid and B12 in methanol/chloroform, drying, and subsequent rehydration by adding 50 wt % of H2O/D2O to the powder.

Among nonpersistent patients, we determined whether a patient made a clinically appropriate switch in the subsequent 90 days by adding a new cholesterol-lowering medication or by increasing the dose of an existing one.

Moreover, this polymeric interfacial catalyst was pH-sensitive and could be easily recycled by adding small amount of acid, and subsequent cycles of alcohol oxidation showed no loss on either conversion or selectivity.

All qPCR data were converted into a logarithmic format by adding 1 to each value and subsequent log10 transformation.