Sentence examples for by adding small volumes from inspiring English sources
Precipitation was initiated by adding small volumes of ethanol.
The initial pH of the working solution was obtained by adding small volumes of 0.5 N HNO3 (for pH 2.0 and 3.0), or acetate buffer (CH3COOH/CH3COONa), for 3.0 < pH < 6.0.
Following proteolytic digestion, the pH of the sample was slowly lowered to <4.0 by adding small volumes (1 µl to 2 µl) of 20% formic acid.
Manganese (Mn2+) titrations were performed by adding small volumes of a concentrated solution (0.5 M) of 99.99% MnCl2 (Sigma-Aldrich, MO) directly to the RNA sample to achieve final concentrations of 5, 10, 20, 40, and 80 μM MnCl2.
The best conditions were attained by adding small aliquots of milk (ten-fold a volume of 0.5 ml added during 5.0 min) to a digestion mixture containing 3.0 ml nitric acid plus 1.0 ml sulfuric acid heated at 105 °C.
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The level II composite was obtained by adding a small volume fraction (vf) of the ball-milled level I composite to Mg using the powder metallurgy route followed by microwave-assisted rapid sintering and hot extrusion.
Chemical reduction, in order to generate the reduced Fe(II)/Fe(III) in the FeS clusters, was performed by adding a small volume of anaerobic sodium dithionite solution to the protein solution under a weak stream of argon gas (20-fold excess dithionite with respect to the protein).
Osmotic stress (0.4 M NaCl) was produced by adding a small volume of concentrated NaCl solution to the yeast culture 10 min before cross-linking with formaldehyde.
To examine the average size of these particles, a bright-field microscopy technique was performed using a Leica DMLM microscope by adding a small volume of spheres on the surface of a microscope slide.
Co II Cbl and Co II Cbi+ were prepared by adding a small volume of saturated HCOOK to degassed solutions of H2OCbl+ and (H2O 2Cbi2+, respectively, and the progress of the reduction was monitored spectrophotometrically.
