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The best conditions were attained by adding small aliquots of milk (ten-fold a volume of 0.5 ml added during 5.0 min) to a digestion mixture containing 3.0 ml nitric acid plus 1.0 ml sulfuric acid heated at 105 °C.
Titrations were performed by adding small aliquots of ANS from a 1 mM stock solution to 2.0 mL of PDC-109 (OD280≅0.05) in the cuvette.
The pH of the solution was adjusted by adding small aliquots of 1N HCl or NaOH.
The pH of the samples was adjusted by adding small aliquots of 0.2 M HCl.
Chromatophores were suspended in 100 mM KCl and 50 mM MOPS (pH 7.0) or 100 mM KCl and 50 mM CHES (pH 9.2) to a reaction center concentration of ∼0.7 mM, and the redox potential was adjusted by adding small aliquots of potassium ferricyanide or sodium dithionite.
Briefly, TP1 polymer (3 mg) was dissolved in 30 mL of 0.1 M NaCl and its pH was adjusted to pH 10.0 with 0.1 N NaOH and then the resulting solution was titrated with 0.1 N HCl by adding small aliquots (50 μL) until pH 3.0 was reached.
Similar(54)
Taking into account the physical mechanism of He dissolution, the sites of the silicate melt structure, where noble gases can be accommodated, drastically increase by adding modest aliquots of H2O.
By adding a small aliquot of sulfide that was stoichiometrically complexed with AgNPs, the nanosilver toxicity to nitrifying organisms was reduced by up to 80%.
Cells were then homogenized with 6% trichloroacetic acid, neutralized by adding a small aliquot of a 1M Tris solution, and centrifuged at 20,800×g for 5 min at 4°C.
Cell viability was determined by adding a small aliquot of cell titer 96 Aqueous One solution cell proliferation MTS assay (Promega), with absorbance measured using a 490 nm filter over time.
The substrate concentrations in the cell were varied by adding different aliquots of the stock solutions (100 mM and 500 mM).
More suggestions(15)
by adding small quantities
by adding small volumes
by adding small perturbations
by adding small rocks
by adding small changes
by analyzing small aliquots
by adding small paintings
by dissolving small aliquots
by adding small amounts
by transferring small aliquots
by adding small touches
by adding different aliquots
by adding fresh aliquots
by removing small aliquots
by pouring small aliquots
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