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The quenching experiment was carried out simply by adding small aliquote (1 10 μL from 100 μM stock solution) of concentrated peptide solution to 1 mL solution containing an appropriate concentration of HSA/BSA (0.5 μM in 20 mM Tris HCl buffer, pH 7.5) taken in 1 cm path length quartz cuvette.
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The pH of the solution was adjusted by adding small aliquots of 1N HCl or NaOH.
The pH of the samples was adjusted by adding small aliquots of 0.2 M HCl.
Precipitation was initiated by adding small volumes of ethanol.
Finish this look off by adding small amounts of hairspray.
Start off by adding small touches to your uniform.
Decorate it by adding small rhinestones, using Sharpies, and adding cell phone charms.
Add detail to the iris by adding small black dots and lines.
Consider adding "leaves" by adding smaller leek stems to the sides of your main stem.
A pH of 6.5 was achieved by adding a small amount of hydrochloric 1N.
A final pH of approximately 8.5 was achieved by adding a small amount of hydrochloric 1N.
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