Sentence examples for by adding serial dilutions from inspiring English sources

The average titer was evaluated on TCR deficient Jurkat 76 cells [24] by adding serial dilutions of concentrated vector to 2.5×105 cells in a 48-well plate (Nunc).

Avidity index (concentration of thiocyanate leading to the dissociation of 50% of the bound IgG in ELISA) was measured by adding serial dilutions of ammonium-thiocyanate after serum incubation (triplicates at half max saturation), leading to partial dissociation of bound antibodies [20].

The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV.

We obtained a standard curve by adding serial dilutions of a standard lysozyme solution (10 to 0.3125 mg/ml) to the bottom row of each plate.

Simulated MERS-CoV positive NPAs were prepared by adding serial dilutions (1 500 000 TCID50/0.1 mL) of MERS-CoV to two different NPAs and 20 TCID50/0.1 mL and 200 TCID50/0.1 mL of MERS-CoV to 10 additional NPAs negative for the above mentioned respiratory viruses.

Chemical compounds to be analyzed were diluted in DMSO; cytotoxic activity was evaluated by adding twofold serial dilutions of the compound to be analyzed, starting at a 1600 μg/mL concentration; the plates were further incubated (24 h).

To evaluate whether inhibition was mediated by single infected cells, we added serial threefold dilutions in replicate wells as depicted schematically in Figure 3A.

As recombinant HB-EGF was added at serial dilutions of known concentration, a standard curve was calculated by use of the graphical package Graphpad Prism.

Compounds were then added in serial dilutions.

Individual mouse serum samples were added in serial dilutions and incubated for 2 hours.

Serum samples were added in serial dilutions starting at a 1/50 dilution and plates were incubated for 2 hours.