Sentence examples for by adding random hexamers from inspiring English sources

With mRNA as the template, first-strand cDNA was synthesized by adding random hexamers and second-strand cDNA was synthesized by adding buffer, dNTPs, DNA polymerase I and RNase H. Double-stranded cDNA was purified using AMPure XP beads.

Then the reaction was added with random hexamers (2.5 ng/μl).

An amount of 100 ng of RNA was converted into cDNA by adding 9.2 ng/uL Invitrogen Random Hexamers, dNTPs (500 uM), and free-RNAse H2O to a final volume of 32.5ul.

cDNA was synthesized by adding 40 ng of random hexamer primers to 1 μg of total RNA in a total volume of 11 μl.

Fragmentation of mRNA was terminated by adding stop solution (Ambion) and used as template to synthesize the first strand cDNA by using random hexamers (Invitrogen).

For gene expression analysis, 1 μg of total RNA was reverse transcribed by using random hexamers.

RNA was transcribed into cDNA by reverse transcription by priming with random hexamers (M-MLTV, Promega, Madison, WI, USA).

Total RNA (1  μg) was primed by random hexamers and converted into cDNA by SuperScript III (Invitrogen).

RNAs were reversed transcribed into cDNA by using random hexamers and Superscript II Plus RNase H− Reverse Transcriptase (Invitrogen).

RNA was reverse transcribed by using random hexamers as primers.

cDNA was generated by using random hexamers and Superscript III (Invitrogen, NY).