In contrast, when Ca2+ was depleted by adding EGTA or BAPTA, the apoptosis rates in both the tps1△ mutant and wild-type cells were decreased.
Start by adding one or two drops.
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Cells were grown in SD+aa with or without uracil, or UTP-depleted by adding 200 mg/l of 6-azauracil to log-phase cells grown in SD+aa and grown for three additional doubling times.
When CDG Ig cells, which accumulate Man7GlcNAc2-PP-dolichol, are permeabilised so that vesicular transport and protein synthesis are abolished, the DLO pool required for Man7GlcNAc2-P generation could be depleted by adding exogenous glycosylation acceptor peptide.
Cells were mock depleted by adding cells to the matrix column without prior incubation with CD25 microbeads.
These three highly abundant lncRNAs could be specifically depleted by adding corresponding specific probes in the RiboZero protocol, thus improving the sequencing depth of the remaining RNAs.
We're by no means creating or depleting another industry.
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Depleting GSH by adding BSO to the culture medium with DPTTS significantly decreased the viability of HOCl fibroblasts (P < 0.01).
