Sentence examples for by adding one volume from inspiring English sources

After the last wash, the supernatant was removed and pure enzyme was eluted by adding one volume of elution buffer (50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole pH 8.0) and mixed thoroughly for 10 min at room temperature.

The stimulation was stopped by adding one volume of 10% trichloroacetic acid, followed by sonication.

In each fraction, a protein elimination step was performed by adding one volume of 8 M guanidium-HCl and two volumes of absolute ethanol [ 6].

After two washes with PBS, cells were trypsinised, pelleted, resuspended in PBS containing 5 m M EDTA and fixed by adding one volume of ethanol (Merck).

DNA was extracted by adding one volume of phenol/chloroform, vortexing and phase separation by centrifugation at 13000 g for 10 min using PhaseLockGel tubes.

The reaction was stopped by cooling each sample and the cellulose was precipitated by adding one volume of water followed by centrifugation (15,000 ×  g for 2 min).

Cells were fixed by adding one tenth volume of concentrated formaldehyde (Sigma-Aldrich) for 10 min and washed with PBS.

Add one volume of 100% cold ethanol.

Add one volume of cold medium and mix well.

For serum-supplemented culture, routinely add one volume of cold FBS.

The suspension was then dounced 20 times using tight pestle followed by adding one third the packed volume of sucrose buffer (20 mM Tris pH 7.6, 15 mM KCl, 60 mM NaCl, 0.34 M Sucrose, 0.15 mM Spermine, 0.5 mM Spermidine).