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Dilution was accomplished by adding methanol to the mark to extract the analyte(s).
Possibly, the amount of free water is reduced by adding methanol to the reaction mixture, since the alcohol can form hydrogen bonds with water.
Then, the cells were harvested by centrifugation, suspended in 30 mL BMMY medium and induced for the expression of recombinant xylanase by adding methanol to a final concentration of 1.0% at 24 h intervals at 30 °C for 72 h.
They accomplished this by adding methanol to a solution of COS in 10% acetic acid followed by addition of different amounts of succinic anhydride dissolved in acetone dropwise at room temperature for 1 h, to obtain CCOS with different substitution degrees.
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To support the effect of Ostwald ripening, size-selective precipitation was carried out by adding methanol dropwise to the reaction mixture at RT until slight cloudiness persisted.
The stable cone regime spray is usually obtained by adding methanol or ethanol to the electrosprayed dipolar fluid, with the role of reducing fluid surface tension.
By adding methanol (100 μL) to the residue, a second methanol extraction was carried out in the same fashion.
The methanol/ammonia solutions were obtained by adding methanol, either 50 or 150 μL, to the NP-5 mixtures, obtaining concentrations of 121 and 359 mM, respectively.
For the cord plasma samples, the proteins were precipitated by adding methanol and centrifuging the mixture prior to injection onto the analytical system.
In addition, no activity decay was observed by adding methanol in the acidic solution indicating its excellent tolerance to methanol oxidation.
The buffer was modified (typically: 5 or 10 mM sodium tetraborate, pH 9.4) by adding methanol (MeOH) or acetonitrile (ACN) in the range up to 40% (v:v).
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