Exact(1)
In all the decomposition experiments pH was kept constant by adding low volumes of a concentrated NaOH or HCl solution correspondingly (1.0 mol L−1).
Similar(59)
Precipitation was initiated by adding small volumes of ethanol.
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However, the traditional methods used to isolate asphaltenes by adding 40 volumes in excess of low boiling point paraffin hydrocarbons can produce a fraction which is contaminated with a significant amount of waxes (Chouparova and Philp 1998; Thanh et al. 1999; Liao et al. 2006; Acevedo et al. 2009; Coto et al. 2011).
To prevent specimen drift during time lapse experiments, whole seedlings in 0.5X MS were mounted by adding 2 volumes of molten 2% low melting point agarose (Research Organics), 2% high resolution 3 1 agarose (FMC) and 0.5X MS salts (Gibco-BRL) at 42°C.
The low molecular weight RNAs were precipitated by adding 3 volumes of ethanol and 1/10 volume of sodium-acetate pH 5.2.
Intracranial volume was calculated by adding gray matter volume, white matter volume, and CSF space volume.
Then, mixed solutions were adjusted to a constant final volume (5 mL) by adding additional volumes of deionized water.
The latter was done by adding 6 volumes of ethylacetate.
The 2,2,2- trifluoroethanol (TFE) experiments were performed by adding increasing volumes of TFE (JT Baker) added to the samples.
After the compounds were completely dissolved, two volumes of Cremophor EL were added and final dosing solution was prepared by adding six volumes of 20% hydroxypropyl β-cyclodextrin.
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