Sentence examples for by adding increasing concentrations from inspiring English sources

We next assessed the direct competition between both proteins by adding increasing concentrations of hnRNP C1 to the U2AF65RRM12-binding reaction.

Accuracy was determined by adding increasing concentrations of purified boPAG67 (1.0, 2.0, 4.0 and 10.0 ng) to buffalo sera containing known PAG concentrations.

Again following washing and re-equilibration, tissues were initially constricted to their maximum by adding increasing concentrations of 5-HT (1 nM to 10 µM).

The ability of synthetic peptidylglycine peptides to inhibit the amidation of Ac-Tyr-Val-Gly was assessed by adding increasing concentrations of competitor to the assay mix.

Competitive curves were obtained by adding increasing concentrations of fatty acids to preformed ANS FABP complexes, and Ki values were calculated from at least four independent experiments (Table  4 and Fig.  7D and E).

Both yeasts were first evaluated for their ability to ferment in the presence of the cocktails A, B, C, and D, formulated by adding increasing concentrations of each inhibitory compound as described earlier (Table  3).

To further confirm these results, we repeated biochemical competition experiments using actin cosedimentation by pre-binding Coro1C R28D or Coro1C 2×KE to actin filaments and adding increasing concentrations of Coro1B.

After equilibration for 30 min, cconcentration-response curve (CRC) for plant extract, histamine or ACh were obtained by cumulatively added increasing concentrations.

and the osmolarity outside of the vesicles was increased by adding increasing sucrose concentrations, ranging from 50 mM to 800 mM, to the Kout > Kin incubation buffer (see 2.5.1.1).. Uptake was performed as described in section 2.5.1.

Anisotropy titrations were carried out by adding increasing hRARα/mRXRα LBDs concentrations to a fixed concentration of tetramethylrhodamine (TAMRA) – SRC-1 NR2 peptide in 10 mM TRIS-HCl (pH 7.5), 150 mM NaCl, 10 mM DTT buffer.

As l-Cys was added with increasing concentrations, an emission band peaked at 580 nm occurred and significantly increased in fluorescence intensity, by which the l-Cys could be sensed optically.