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Briefly, cells were crosslinked by adding formaldehyde to 1% final concentration directly to the cell culture medium and incubated 10 min at room temperature.
In brief, cells were fixed by adding formaldehyde to 1% final concentration for 10 min at room temperature.
Proteins were cross-linked to DNA in 5×107 cells by adding formaldehyde to a final concentration of 1% and incubating for 10 minutes at room temperature.
Yeast cells were fixed by adding formaldehyde to a final concentration of 5% and incubating at least 2 h at room temperature with gentle shaking.
Proteins and nucleic acids were crosslinked by adding formaldehyde to a final concentration of 1% and incubated for 15 minutes at 37°C.
Cross-linking of proteins to the DNA of cells (isolated from spleen or cell culture) was achieved by adding formaldehyde to a final concentration of 1% for 15 min at 37°C with occasional inversion.
Similar(39)
A volume of 40 mL YPD culture from each one (O.D.600 ≈ 0.5-0.8 0.5-0.8t aside, and proteins wase crosetlinked to their taside sites in vivo by anding formaldehyde at a final concentration of 1%.
Nuclear proteins were cross-linked to DNA by adding formaldehyde directly to the medium to a final concentration of 1% for 8 min at room temperature.
Then nuclear proteins were cross-linked to DNA by adding formaldehyde directly to the medium to a final concentration of 1% following incubation for 10 min at room temperature on a rocking platform.
Protein complexes were cross-linked to DNA by adding formaldehyde directly to tissue culture medium to a final concentration of 1%.
Cells were cross-linked by adding formaldehyde directly to the medium to a final concentration of 1%, at room temperature.
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