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No competition was observed by adding equivalent amounts of non-specific competitors.
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The contribution of the dilution effect to the fluorescence signal was corrected by using equivalent liposome or cell suspensions and adding equivalent amounts of DMSO solvent (final concentration did not exceed 0.6%).
Instead, we relied on adding equivalent amounts of RNA template in each experiment.
To each group we added equivalent amounts of BOD-CH.
The method was conducted by adding electrically equivalent amounts of SDS and PAH followed by feeding air bubbles.
The effect of MVs on DC was determined by adding equal amounts in protein equivalents of MVs for 16 hours at day 8 of the DC culture.
Several of these compounds, when added in equivalent amounts, produce 1.2 M LiF solutions in DME, an increase in solubility of LiF by six orders of magnitude.
Briefly, 1 μl of oligo dT12 18 (Life Technologies-Gibco BRL, Gaithersburg, MD, USA) was added to equivalent amounts of total RNA from control and diabetic human atrial appendages.
Concentrations of the reducing sugar in inulin were determined by the dinitrosalicylic acid method [ 61], and the total sugar was firstly hydrolyzed by 0.2 M H2SO4 at 100°C for 1 h, then measured by the methods of Miller [ 61] after adding an equivalent amount of 0.4 M NaOH.
The challenge model involved restricting consumption of the TMR to 0.5 of ad libitum intake for 24 h (day −1; prechallenge) followed by adding barley grain (amount equivalent to 0.25 of DMI) directly to the rumen prior to feeding the TMR (day 0; challenge day).
Adapters were prepared by adding equimolar amounts of both strands.
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