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Adapters were prepared by adding equimolar amounts of both strands.
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Ion conductive properties of TNT/BMImTFSA were studied by AC impedance method after adding equimolar amount of LiTFSA to BMImTFSA.
The precursor SNAP-acid (4 mg/mL) was activated by adding an equimolar amount of tetrabutylammonium hydroxide (TBAH) and dissolved in the respective solvent.
The labeling reaction was allowed to proceed while the mixture was slowly and constantly mixed at 4 °C overnight, and unreacted dye was quenched by adding an equimolar amount of glutathione.
Next, unlabeled HAD peptide was added in equimolar amounts with respect to the initial complex concentration.
The LF or LF-TcdB were added in equimolar amounts with protective antigen as previously described [24].
The oligonucleotides were added in equimolar amounts into 100 μL 1 × Gold Buffer (Invitrogen) and heated to 94°C for 15 min, followed by three cycles with 58°C for 10 min and 70°C for 5 min.
Routinely, reactions were carried out using 20-100 μM IC in combination with various mediators that were added in equimolar amounts or in a molar ratio of 1 10.
In similar reporter assays, HEK-TLR3 cells were transfected with reporter plasmids for IFN-β, NFκB, CCL5, and the internal control β-gal, and expression plasmids pcDNA3-I329L or pcDNA3 (negative control), keeping the total amounts of DNA and equimolar ratios constant in all assays by adding the appropriate amount of empty vector.
Add fragments in equimolar amounts.
The PCR fragments were added either in equimolar amounts or as a 2-fold excess of the vector (50 100 ng).
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