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The same sets of experiments were repeated by adding equal concentration of scale inhibitor with each corrosion inhibitor.
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The siRNA complex was formed in the 48 well plate by adding equal volumes of the stock concentration of siRNA and OptiMEM and incubating at room temperature in a laminar flow hood.
Multiplex primer mixes were made by adding equal amounts of all primers in that set at a concentration of 100 µM, and then adding either 4.0 µl (Amplification Sets 1 and 3) or 4.4 µl (Amplification Set 2) to each 20 µl 1st-round PCR tube, to give a final concentration of 1 µM.
Reactions were stopped by adding equal volume of 2X SDS PAGE sampling buffer.
Every tube, within a metapopulation, was then subsequently mixed together by adding equal volumes of culture.
Globin reduction PNA master mix was prepared by adding equal amounts of the globin PNA stocks.
Subsequently the media was removed and cells were lysed by adding equal volume of dimethyl sulfoxide.
The reaction was quenched by adding an equal volume of water and incubation for 30 min. Sample was dried by spin-vap and resuspended in 0.1% formic acid for a final concentration of 1 mg/ml protein.
The enzymatic reactions were stopped by adding an equal amount of ice-cold methanol containing 20 μM of the internal standard 10-methoxy-carbamazepine, followed by 1 1 dilution with deionized water for a final methanol concentration of 25%.
The reaction was stopped by adding an equal volume of acidic (2N HCl) stop solution.
Purified PCR products were denatured by adding an equal volume of 0.6 M NaOH.
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