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The digestion was stopped by adding double volume of HBSS supplemented with 10% FBS.
Prolamin was precipitated by adding double volume of acetone (VWR Scientific, Suwanee, GA, USA) to the supernatant.
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The volume was made to 50 ml by adding double-distilled water and used for analysis.
The oligosaccharides were eluted from the paper by adding double-distilled water (2 × 500 μL).
Put the chickpeas and remaining baking soda in a medium saucepan, add double the volume of cold water and bring to a boil.
Intracranial volume was calculated by adding gray matter volume, white matter volume, and CSF space volume.
Samples were also enriched by adding an equal volume of double strength selenite broth, followed by overnight incubation at 37°C.
The reaction mixture was added in double the volume of the media and was kept in dark for 45 min and then the reaction was stopped by adding 1/10 volume of 1 N HCl to each well.
The reaction was stopped by adding 10x volume cold PBS.
The reaction was stopped by adding one volume of FBS.
The volume was adjusted to 600 µl by water followed by adding an equal volume of phenol, pH 4.2.
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