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Inhibition of the radioligand was performed by adding different concentration series (1 500 nM) of unlabelled compounds (A3R antagonists) each after visual binding equilibrium.
By adding different concentration of formamide in the [Na+] 0.25 M buffer, we observed that the HRM curves for both the probes and templates were shifted horizontally towards lower temperature range, the amount shifted horizontally was directly proportional to the amount of formamide added, and the addition of formamide did not interfere with the Na+ salt-induced effect, as shown in Figure 2(a).
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Fig. 2 Pigment extracted from the mature cells by adding different concentrations of Triton X-100.
Fig. 1 Pigment extracted from the fermentation broths by adding different concentrations of Triton X-100.
Several different phase viscosities were used and the effect of interfacial tension was examined by adding different concentrations of sodium dodecyl sulphate in the aqueous phase.
Experiments were conducted by adding different concentrations of sulfurous acid to a CO2-saturated solution at 25 °C under different pH conditions.
The influence of pH has also been studied by adding different concentrations of sodium hydroxide to ethanolic solutions (ethanol p.a. + water + sulphate).
For the first time, we find that thickness-tunable interface films could be generated on LiCoO2 surface by adding different concentrations of BMP additives in electrolyte.
Ceramic samples were produced by adding different concentrations of PG (5, 7.5, and 10 wt%) to natural clay, and sintering at 950, 1050, and 1150 °C.
Binding of [125I]-AB-MECA to the seeded cells was realized by adding different concentrations (0.05 5 nM) to fully cover the concentration span needed for proper affinity estimation.
As shown in Figure 5a, with 100 nM CdTe QDs as a control PCR that inhibits the reaction entirely, by adding different concentrations of BSA, nonspecific amplification bands reoccurred despite a high annealing temperature.
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