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The substrate concentrations in the cell were varied by adding different aliquots of the stock solutions (100 mM and 500 mM).
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The best conditions were attained by adding small aliquots of milk (ten-fold a volume of 0.5 ml added during 5.0 min) to a digestion mixture containing 3.0 ml nitric acid plus 1.0 ml sulfuric acid heated at 105 °C.
Taking into account the physical mechanism of He dissolution, the sites of the silicate melt structure, where noble gases can be accommodated, drastically increase by adding modest aliquots of H2O.
Titrations were performed by adding small aliquots of ANS from a 1 mM stock solution to 2.0 mL of PDC-109 (OD280≅0.05) in the cuvette.
The experiment was repeated three times by adding fresh aliquots of each conjugate and reoxygenating.
The pH of the solution was adjusted by adding small aliquots of 1N HCl or NaOH.
The pH of the samples was adjusted by adding small aliquots of 0.2 M HCl.
Moreover, we demonstrate the pH responsiveness of these interfacial multilayers by adding aliquots of NaOH to the acidic water subphase surrounding the bubbles and droplets.
(a) Calibration curve obtained by adding aliquots of saturated NO solution (1.87 mM) to PBS containing DAF-FM indicator.
Parallel experiments were also conducted by adding aliquots from 0.25 M NaHCO3 pre-adjusted to pH 7.4, to the same final concentrations as those of CaCl2 and Na2HPO4.
Pulse labelling of photoautotrophic Synechocystis cultures was performed by adding aliquots of a saturated solution of 13C labelled NaHCO3 to a final concentration of 2% (w/w).
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