Dilutions were made by adding appropriate volumes of the stock solution to be included in 100 ml of water.
To get lower concentrations, the 5-mM samples were diluted by adding appropriate volumes of the surfactant stock solution.
After protein concentration determination, total cell lysates were equalized by adding appropriate volumes of Laemmli's sample buffer (Laemmli, 1970) before analyzing by SDS/PAGE and immunoblotting.
Calibrator and quality control samples were prepared by adding appropriate volumes of standard PCZ solution in acetonitrile to drug free plasma.
Standard curves were created by adding appropriate volumes of each reference DNA solution (porcine, bovine, ovine, or caprine) in heparinase digestion buffer to achieve concentrations of 0.1, 1, 10, 100, and 1,000 pg DNA/μL.
The seed cultures for transformants expressing an empty vector, chimeric CBHI, EGII, and CBHII, were first adjusted to the same OD value, and then used to prepare the following four types of cell mixtures by adding appropriate volumes of seed cultures (cells) to 150 mL of mineral medium containing 4 g Avicel.
Batch cultivation was carried out in a 500-mL baffled flask containing 150 mL of BBM supplemented with 10 g of a carbon source (pure glucose or the same amount of glucose by adding appropriate volumes of the RSH), and 3 g of yeast extract per liter was used as a nitrogen source instead of NaNO3.
The solution was prepared by accurately adding appropriate volume of each of the prepared standard solution.
They are made in all colours by adding appropriate pigments.
To account for delamination effects, displacement field is enriched by adding appropriate terms.
