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The assay was performed by adding appropriate dilutions of 20 μl of the secretome to pNP plates that had been previously preincubated at 37°C.
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Dilutions were made by adding appropriate volumes of the stock solution to be included in 100 ml of water.
Calibration standards were prepared by adding appropriate amounts of the stock solutions to flavonoid and isoflavone-free human urine and then performing serial dilutions with additional blank urine sample to obtain different concentrations.
They are made in all colours by adding appropriate pigments.
The reactions were started by adding different dilutions of the cell-free extract.
Add appropriate verbal exclamations.
Add appropriate wall decor.
Phospho-PYK2 antibody was added at appropriate dilution (1 100) on slides and left for 30 min in a humid atmosphere at room temperature.
The recipient population was enumerated by plating appropriate dilutions on the BG medium without any antibiotics.
Viable counts were determined by plating appropriate dilutions onto LB plates.
Standard working solutions were obtained by making appropriate dilutions.
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