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Two concentrations of Mg (1.9 and 4.3 g/kg of dry matter) and 3 concentrations of K (19, 28, and 37 g of K/kg of dry matter) were obtained by adding appropriate amounts of MgO and KHCO3 to the diet.
The vanadium oxides supported on SBA-15 were prepared by adding appropriate amounts of butylammonium decavanadate [31] to a suspension of functionalized SBA-15 in water.
For the DLLME, an aliquot of 5 ml samples containing 2, 10 and 30 μg ml− 1 of morphine, codeine and methadone were prepared and pH of the samples was adjusted at 9.8 by adding appropriate amounts of sodium carbonate (10%w/v).
The Sb electrolyte is an aqueous solution consisted of 0.02 mol L−1 SbCl3, 0.1 mol L−1 C6H8O7·H2O, and 0.05 mol L−1 K3C6H5O7·H2O, and the Co electrolyte is an aqueous solution consisted of 0.05 mol L−1 CoSO4 and 0.25 mol L−1 H3BO3, the pH value of both the electrolytes was adjusted to about two by adding appropriate amounts of 5 M H2SO4 solutions.
Total DNA in each transfection was adjusted by adding appropriate amounts of pcDNA3 empty vector.
Electroneutrality and physiological ionic strength of ∼0.150 M for the CaB simulations and 0.1 M for the free Ca2+ simulations were reached by adding appropriate amounts of Na+ and Cl− ions to the simulation boxes (as detailed in Table 3).
Similar(46)
It is found that the gradation optimization by adding appropriate amount of the coarse virgin aggregate (VA) can greatly improve the mechanical strength and pavement performances of CRMs.
The electrolyte consists of 1 M CoSO4·7H2O and the initial pH of this solution was adjusted to 6 by adding appropriate amount of H2SO4.
The purification procedures of the samples were carried out by adding appropriate amount of ethanol into the samples and centrifuging at 4000 rpm for 10 min. After that, the precipitates were collected and washed twice with chloroform to remove precursor and surfactant residuals.
Virus binding to the glycan-coated wells was performed as described previously [17] by adding appropriate amount of virus to each well after diluting in PBS containing 1% BSA and incubating overnight at 4°C.
Supernatant was collected, and alkanes were extracted by adding appropriate amount of chloroform and 50 mM Tris Cl (pH7.5).
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