An additional phenol/chloroform extraction was performed by adding an equal volume of phenol/chloroform to the isolated aqueous fraction.
The labeling reaction was stopped by adding an equal volume of 1% BSA.
RCOOH can be converted completely to the ion RCOO− by adding an equal number of molecules of a base such as sodium hydroxide (NaOH).
After 15 min at 55 °C, RNA was extracted by adding an equal volume of phenol: chloroform: isoamyl alcohol (25:24:1, Sigma, P3803).
The reaction was stopped by adding an equal volume of 100 mM sodium hydroxide to the reaction mixture after 45 min or 90 min, respectively.
By adding an equal amount of weight to the bare foot, they could learn whether barefoot running really was physiologically more efficient than wearing shoes.
The staining reaction was stopped by adding an equal amount of 1% bovine serum albumin (BSA), and exosomes were washed in PBS and pelleted by ultracentrifugation (1 h at 100,000 g).
Traditional precipitation of BSA by adding an equal volume of organic solvent, often successfully used with conventional HPLC-PDA, was found insufficiently robust when novel fused-core HPLC and/or UPLC-MS methods were used.
A reaction solution containing digoxin as an internal standard and ginsenosides as substrates and products was extracted by adding an equal volume of n-butanol.
All the reactions were stopped and extracted by adding an equal volume of n-butanol with digoxin as an internal standard.
The volume was adjusted to 600 µl by water followed by adding an equal volume of phenol, pH 4.2.
