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In order to improve the thermal stability and carbon-deposition resistance of the Ni/γ-Al2O3 during the reforming of methane with carbon dioxide, the catalyst was modified by adding an alkaline earth (CaO).
TB assay was developed by adding an alkaline phosphatase-conjugated detection antibody and substrate.
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By adding an ascot!
The amount of nuclear and cytoplasmic p65 was detected by adding a secondary antibody followed by alkaline-phosphatase-conjugated antibody.
Subsequently, in the same well, the quantity of enzyme is measured by adding a complex IV specific antibody conjugated with alkaline phosphatase.
The outline of the alkaline plume is visualized by adding a pH indicator into both solutions.
He then added an alkaline, sodium carbonate, and rendered the smooth modern cocoa.
Bound probes were later visualized by adding the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) then nitroblue tetrazolium (NBT).
Following another PBST wash, plates were developed by adding the alkaline phosphatase yellow (pNPP) liquid substrate for ELISA (Sigma).
Both ELISAs were developed by adding the alkaline phosphatase substrate p-nitrophenyl phosphate (Sigma), and the OD was monitored at 405 nm using a MRX Revelation ELISA Reader DYNEX Technologiess, Chantilly, VA).
Plates were then washed with PBST, and the signal was developed by adding the Alkaline Phosphatase Yellow (pNpp) Liquid Substrate System for ELISA (Sigma).
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