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Parallel experiments were also conducted by adding aliquots from 0.25 M NaHCO3 pre-adjusted to pH 7.4, to the same final concentrations as those of CaCl2 and Na2HPO4.
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These dilution tubes were kept at 2 4°C until Monday morning when the prepared faecal samples were spiked by adding aliquots of peptone saline water from selected dilution tubes.
The switch in arsenic speciation from oxyanionic to sulfoxyanionic forms was reversible and triggered by adding aliquots of a 1 mM sodium bisulfide buffer to solutions containing about 2 to 15 μM arsenite from NaAsO2.
(a) Calibration curve obtained by adding aliquots of saturated NO solution (1.87 mM) to PBS containing DAF-FM indicator.
Titrations were performed by adding small aliquots of ANS from a 1 mM stock solution to 2.0 mL of PDC-109 (OD280≅0.05) in the cuvette.
ATPase activity was determined by adding 50 μL aliquots from the assay described above to 1 mL of ATPase assay buffer.
The best conditions were attained by adding small aliquots of milk (ten-fold a volume of 0.5 ml added during 5.0 min) to a digestion mixture containing 3.0 ml nitric acid plus 1.0 ml sulfuric acid heated at 105 °C.
The substrate concentrations in the cell were varied by adding different aliquots of the stock solutions (100 mM and 500 mM).
Taking into account the physical mechanism of He dissolution, the sites of the silicate melt structure, where noble gases can be accommodated, drastically increase by adding modest aliquots of H2O.
The experiment was repeated three times by adding fresh aliquots of each conjugate and reoxygenating.
The pH of the solution was adjusted by adding small aliquots of 1N HCl or NaOH.
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