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Finally, we also find that the wrapping process can be completely retarded by adding a substrate below the graphene flake.
After washing of unbound ACE, plate-bound ACE activity was measured by adding a substrate for ACE (Hip-His-Leu) directly into wells [33], [36].
A color reaction was developed by adding a substrate solution containing 3,3′,5,5′-tetramethylbenzidine 3,3′,5,5′-tetramethylbenzidine.
This specific compound or protein can then be quantified by adding a substrate for the reporter enzyme and measuring the concentration of the product [ 22].
The labelled secondary antibody was visualised by adding a substrate containing diaminobenzadine (Zymed), and sections were counter-stained, dehydrated and mounted.
After 3 washes, color development was initiated by adding a substrate solution and was monitored at 450 nm using a microplate reader (Emax, Molecular Devices).
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By adding a porous substrate with a high internal surface in the hot zone of the reactor, the consumption of specific species is enhanced, revealing the effective precursors of the solid (e.g., HBCl2 giving rise to boron).
Residual enzyme activity was evaluated (spectro-photometrically at 513 nm and 25 °C) by adding a starch substrate to the test sample and monitoring the kinetics of color formed from the reducing action of hemiacetal groups (librated from starch hydrolysis) on 3,5-dinitrososalicylic acid.
Following aspiration and washes, the chemokine of interest was detected by adding a chromogenic substrate followed by a stop solution.
The specific protein/auto-antibody/conjugate complexes are revealed by adding a peroxidase substrate which results in a colorimetric reaction.
After washing away unbound HRP conjugate, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops in a spectrophotometer.
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