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Even The Wire, the holy grail of critically acclaimed dramas, started getting a little crazy in its fifth season by adding a serial killer, the death knell of many a procedural drama.
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Antibody titer changes were investigated as well: after centrifugation the supernatant was stored at 4°C and anti-A titers were measured the following day by adding A erythrocytes in serial plasma dilutions and compared to the original titer.
The integrity of a Modbus message is validated by using the TCP layer for Modbus/TCP or by adding a control field (cyclic redundancy check or CRC) for Modbus/Serial.
By adding an ascot!
We obtained a standard curve by adding serial dilutions of a standard lysozyme solution (10 to 0.3125 mg/ml) to the bottom row of each plate.
Chemical compounds to be analyzed were diluted in DMSO; cytotoxic activity was evaluated by adding twofold serial dilutions of the compound to be analyzed, starting at a 1600 μg/mL concentration; the plates were further incubated (24 h).
The average titer was evaluated on TCR deficient Jurkat 76 cells [24] by adding serial dilutions of concentrated vector to 2.5×105 cells in a 48-well plate (Nunc).
A series of mixtures composed of non-fucosylated and fucosylated anti-CD20s at different ratios was prepared by adding serial 3-fold amounts of fucosylated anti-CD20 to a fixed amount (0.11 μg/mL) of non-fucosylated anti-CD20, and ex vivo B-cell depletion activities of the mixtures were measured (Fig. 3b, donor 3).
Avidity index (concentration of thiocyanate leading to the dissociation of 50% of the bound IgG in ELISA) was measured by adding serial dilutions of ammonium-thiocyanate after serum incubation (triplicates at half max saturation), leading to partial dissociation of bound antibodies [20].
The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV.
Simulated MERS-CoV positive NPAs were prepared by adding serial dilutions (1 500 000 TCID50/0.1 mL) of MERS-CoV to two different NPAs and 20 TCID50/0.1 mL and 200 TCID50/0.1 mL of MERS-CoV to 10 additional NPAs negative for the above mentioned respiratory viruses.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com